Morata Gines, Herrera Salvador C
Centro de Biología Molecular, CSIC-UAM, Madrid, Spain.
Fly (Austin). 2010 Jul-Sep;4(3):226-9. doi: 10.4161/fly.4.3.11516. Epub 2010 Jul 1.
We have generated wing disc compartments that contain marked fast growing M+ clones surrounded by slow dividing M/+ cells. Under these conditions the interactions between fast and slow dividing cells at the clone borders frequently lead to cell competition. However, our assay suppressing apoptosis indicates that cell competition plays no major role in size control. We argue that cells within a compartment proliferate according to their genotype independently of each other and that their contribution to the final structure will depend solely on their proliferation rate. This model is supported by a computer simulation that predicts values similar to those found experimentally. Our results on the growth of M+ clones within compartments and on the expression of developmental genes like vestigial and wingless suggest the existence of a non-cell autonomous mechanism that functions at the level of the entire cell population. It measures the population size in each moment, determines the corresponding expression levels of developmental genes and establishes the time to arrest growth.
我们已经构建了翅盘隔室，其中包含有标记的快速生长的M+克隆，其周围是缓慢分裂的M/+细胞。在这些条件下，克隆边界处快速分裂和缓慢分裂细胞之间的相互作用经常导致细胞竞争。然而，我们抑制细胞凋亡的实验表明，细胞竞争在大小控制中并不起主要作用。我们认为，隔室内的细胞根据其基因型相互独立地增殖，并且它们对最终结构的贡献将仅取决于它们的增殖速率。该模型得到了计算机模拟的支持，该模拟预测的值与实验中发现的值相似。我们关于隔室内M+克隆生长以及诸如vestigial和wingless等发育基因表达的结果表明，存在一种非细胞自主机制，该机制在整个细胞群体水平上起作用。它在每个时刻测量群体大小，确定发育基因的相应表达水平，并确定停止生长的时间。
