Lemriss S, Laurent F, Couble A, Casoli E, Lancelin J M, Saintpierre-Bonaccio D, Rifai S, Fassouane A, Boiron P
UMR CNRS Ecologie Microbienne (Center for Microbial Ecology), Labratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, Université Claude Bernard Lyon, France.
Can J Microbiol. 2003 Nov;49(11):669-74. doi: 10.1139/w03-088.
The purpose of this work was to screen clinical isolates of actinomycetes producing nonpolyenic antifungals. This choice was made to limit the problem of rediscovery of well-known antifungal families, especially polyenic antifungals. One hundred and ten strains were tested, using two diffusion methods and two test media, against three yeast species and three filamentous fungi. Among 54 strains (49%) showing antifungal activity, five strains belonging to the genus Streptomyces were active against all test organisms and appeared promising. These results indicate that clinical and environmental isolates of actinomycetes could be an interesting source of antifungal bioactive substances. The production of nonpolyenic antifungal substances by these five active isolates was investigated using several criteria: antibacterial activity, ergosterol inhibition, and UV-visible spectra of active extracts. One active strain responded to all three selection criteria and produced potentially nonpolyenic antifungal metabolites. This strain was retained for further investigation, in particular, purification, structure elucidation, and mechanism of action of the active product.
这项工作的目的是筛选产生非多烯类抗真菌剂的放线菌临床分离株。做出这一选择是为了限制重新发现知名抗真菌家族（尤其是多烯类抗真菌剂）的问题。使用两种扩散方法和两种测试培养基，对110株菌株针对三种酵母菌种和三种丝状真菌进行了测试。在显示出抗真菌活性的54株菌株（49%）中，五株属于链霉菌属的菌株对所有测试生物体均有活性，且表现出前景。这些结果表明，放线菌的临床和环境分离株可能是抗真菌生物活性物质的一个有趣来源。使用几个标准对这五株活性分离株产生非多烯类抗真菌物质的情况进行了研究：抗菌活性、麦角固醇抑制以及活性提取物的紫外可见光谱。一株活性菌株符合所有三个选择标准，并产生了潜在的非多烯类抗真菌代谢产物。该菌株被保留用于进一步研究，特别是活性产物的纯化、结构解析和作用机制。
