Langhansová L, Konrádová H, Vanĕk T
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of Czech Republic, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
Plant Cell Rep. 2004 May;22(10):725-30. doi: 10.1007/s00299-003-0750-2. Epub 2004 Jan 21.
Embryogenic culture was initiated from mature zygotic embryos of Panax ginseng. Multiple somatic embryos formed and proliferated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2.26 microM) and kinetin (0.046 microM). Mature as well as immature somatic embryos grew into plantlets lacking roots on the same media. Histomorphological analysis of somatic embryos treated with abscisic acid (ABA) and polyethylene glycol (PEG 4000) showed a slight improvement in the root meristem organization of torpedo-stage embryos (embryos were more compact and their cells exhibited a lower degree of vacuolation). Shoot regeneration of non-treated somatic embryos was 31% while that for somatic embryos treated with PEG 4000 and ABA was 70%. Moreover, 75% of plants regenerated from PEG- and ABA-treated embryos formed roots while plants from non-treated embryos did not form roots.
人参胚性培养起始于成熟合子胚。在添加了2,4 - 二氯苯氧乙酸（2.26微摩尔）和激动素（0.046微摩尔）的Murashige和Skoog培养基上形成并增殖了多个体细胞胚。成熟和未成熟的体细胞胚在相同培养基上长成无根的小植株。用脱落酸（ABA）和聚乙二醇（PEG 4000）处理体细胞胚的组织形态学分析表明，鱼雷期胚的根分生组织组织化有轻微改善（胚更紧凑，其细胞液泡化程度较低）。未处理的体细胞胚的芽再生率为31%，而用PEG 4000和ABA处理的体细胞胚的芽再生率为70%。此外，从经PEG和ABA处理的胚再生的植株中有75%形成了根，而从未处理的胚再生的植株没有形成根。
