Gorpenchenko T Y, Kiselev K V, Bulgakov V P, Tchernoded G K, Bragina E A, Khodakovskaya M V, Koren O G, Batygina T B, Zhuravlev Yu N
Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 159 Stoletija Street, Vladivostok 690022, Russia.
Planta. 2006 Feb;223(3):457-67. doi: 10.1007/s00425-005-0102-2. Epub 2005 Sep 1.
Expression of the Agrobacterium rhizogenes rolC gene in Panax ginseng callus cells results in formation of tumors that are capable to form roots. The selection of non-root forming tumor clusters yielded the embryogenic 2c3 callus line, which formed somatic embryos and shoots independently of external growth factors. Although the 2c3 somatic embryos developed through a typical embryogenesis process, they terminated prematurely and repeatedly formed adventitious shoot meristems and embryo-like structures. A part of the shoots and somatic embryos formed enlarged and fasciated meristems. This is the first indication of the rolC gene embryogenic effect and, to our knowledge, the first indication that a single gene of non-plant origin can induce somatic embryogenesis in plants.
发根农杆菌rolC基因在人参愈伤组织细胞中的表达导致形成能够生根的肿瘤。选择不形成根的肿瘤团块得到了胚性2c3愈伤组织系，该系可独立于外部生长因子形成体细胞胚和芽。尽管2c3体细胞胚通过典型的胚胎发生过程发育，但它们过早终止并反复形成不定芽分生组织和胚状结构。一部分芽和体细胞胚形成了增大的和扁化的分生组织。这是rolC基因胚性效应的首次迹象，据我们所知，这也是非植物来源的单个基因能够诱导植物体细胞胚发生的首次迹象。
