Smith Jena L, Garry Vincent F, Rademaker Alfred W, Martin Renée H
Department of Medical Genetics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Mol Reprod Dev. 2004 Mar;67(3):353-9. doi: 10.1002/mrd.20022.
This study examined the effect of paternal environmental exposure to pesticides on the frequency of aneuploidy in human sperm. To determine if the chromosome number in germ cells was altered by paternal exposure, multicolor fluorescence in situ hybridization (FISH) analysis was utilized to measure aneuploidy frequencies in the sperm of 40 men (20 exposed, 20 controls). Samples were coded for "blind analysis" to eliminate scorer bias. Aneuploidy and diploidy frequencies were assessed for chromosomes 13, 21, X, and Y. A minimum of 10,000 sperm was scored per donor per chromosome probe with a total of 809,935 sperm scored. Hybridization efficiency was 99%. There were no significant differences in aneuploidy or diploidy frequencies between exposed and control groups, suggesting that the pesticides did not increase the risk of numerical chromosomal abnormalities in these men.
本研究调查了父亲环境暴露于农药对人类精子非整倍体频率的影响。为了确定生殖细胞中的染色体数目是否因父亲暴露而改变，采用多色荧光原位杂交（FISH）分析来测量40名男性（20名暴露者，20名对照者）精子中的非整倍体频率。样本进行了编码以进行“盲法分析”，以消除评分者偏差。评估了13号、21号、X和Y染色体的非整倍体和二倍体频率。每个供体每个染色体探针至少对10,000个精子进行评分，共评分809,935个精子。杂交效率为99%。暴露组和对照组之间的非整倍体或二倍体频率没有显著差异，这表明这些农药并未增加这些男性出现染色体数目异常的风险。
