Kosenko E A, Venediktova N I, Kaminskiĭ Iu G
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Puschino, Moscow Region, 142290 Russia.
Izv Akad Nauk Ser Biol. 2003 Sep-Oct(5):542-6.
The effect of ammonia and calcium on the activity of monoamine oxidase (MAO) was studied. The enzyme activity in non-synaptic brain mitochondria isolated from rats administered with ammonium acetate was assayed by release of H2O2 using spectrophotometry. The effect of calcium on MAO was assayed directly after addition of Ca2+ to the non-synaptic mitochondria isolated from the forebrain of the control rats. Both ammonium acetate injection in vivo and Ca2+ addition in vitro stimulated the activity of MAO A but not that of MAO B in mitochondria. This is the first evidence that ammonia and Ca2+ regulate MAO A in non-synaptic mitochondria in the forebrain and can contribute to oxidative stress in the neurons via MAO A activation.
研究了氨和钙对单胺氧化酶（MAO）活性的影响。通过分光光度法检测过氧化氢的释放，测定了从给予醋酸铵的大鼠分离出的非突触性脑线粒体中的酶活性。在向从对照大鼠前脑分离出的非突触性线粒体中加入Ca2+后，直接测定钙对MAO的影响。体内注射醋酸铵和体外添加Ca2+均刺激了线粒体中MAO A的活性，而非MAO B的活性。这是首个证据表明氨和Ca2+可调节前脑非突触性线粒体中的MAO A，并可通过MAO A激活导致神经元氧化应激。
