Minvielle F, Coville J L, Krupa A, Monvoisin J L, Maeda Y, Okamoto S
Génétique factorielle, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France.
Genet Sel Evol. 2000 May-Jun;32(3):289-302. doi: 10.1186/1297-9686-32-3-289.
DNA fingerprints of Japanese quail male and female pure line breeders were obtained with probes 33.6, 33.15, and R18.1 and they yielded a total of 59 scoreable bands. Bandsharing (0<BS<1) was calculated within and between six quail lines of two origins, and under reciprocal recurrent (AA and BB), within-line (DD and EE) or no (PP and FF) selection. Twenty one pair types were compared. BS was 0.30 higher within line than between lines. BS with the control line was smaller for reciprocal recurrent selection lines than for lines under individual selection. Bandsharing between the two reciprocal recurrent selection lines was 0.19 lower than between lines under individual selection. These results indicate that the two selection methods had different effects on the genetic constitution of the lines, in agreement with previous observations made from the analysis of biochemical polymorphisms with the same set of birds. Egg production and weight traits of pure and crossbred progeny from fingerprinted quail were obtained and compared, and a linear relationship with the measure of bandsharing was estimated. No significant regression coefficient of performance on BS was found over all progeny genetic types. Heterosis from individual matings could also be estimated under the two selection methods since the same birds were parents of both pure and crossbred performance-tested quail. The association of heterosis with the difference between BS of parents of the purebreds and BS of parents of their half-sib crossbreds was favourable and significant for early production traits in lines DD and EE, but no relationship was found in lines AA and BB. These results indicate that the high level of heterosis obtained through reciprocal recurrent selection, and the heterosis observed under within-line selection may have, partly at least, a different genetic determinism. Therefore, the relationship of heterosis with BS may also depend on the past history of selection in the lines.
利用探针33.6、33.15和R18.1获得了日本鹌鹑纯系雌雄育种者的DNA指纹图谱，共产生了59条可计分条带。计算了两个来源的六个鹌鹑品系内部和之间以及在互交轮回（AA和BB）、系内（DD和EE）或无（PP和FF）选择条件下的条带共享率（0<BS<1）。比较了21种配对类型。品系内的条带共享率比品系间高0.30。互交轮回选择品系与对照品系的条带共享率低于个体选择品系。两个互交轮回选择品系之间的条带共享率比个体选择品系之间低0.19。这些结果表明，这两种选择方法对品系的遗传构成有不同影响，这与之前对同一批鸟类进行生化多态性分析得出的观察结果一致。获得并比较了指纹鹌鹑纯系和杂交后代的产蛋量和体重性状，并估计了与条带共享率测量值的线性关系。在所有后代遗传类型中，未发现性能对条带共享率的显著回归系数。由于同一批鸟类是纯系和杂交性能测试鹌鹑的亲本，因此也可以在两种选择方法下估计个体交配的杂种优势。杂种优势与纯种亲本的条带共享率和其半同胞杂交种亲本的条带共享率之间的差异呈正相关，对于DD和EE品系的早期生产性状具有显著意义，但在AA和BB品系中未发现相关性。这些结果表明，通过互交轮回选择获得的高水平杂种优势以及系内选择观察到的杂种优势可能至少部分具有不同的遗传决定因素。因此，杂种优势与条带共享率的关系也可能取决于品系过去的选择历史。
