Ali A M, Qureshi A H, Rafi S, Roshan E, Khan I, Malik A M, Shahid S A
Department of Microbiology, Army Medical College and Military Hospital, Rawalpindi.
J Pak Med Assoc. 2003 Nov;53(11):517-20.
To determine the frequency of Campylobacter jejuni infection in children suffering from diarrhoea/dysentery in the Department of Microbiology, Army Medical College and Military Hospital, Rawalpindi, from 29 August 2002 to 29 November 2002.
The study was carried out on one hundred stool samples of children up to the age of twelve years admitted with diarrhoea/dysentery in Military hospital, Rawalpindi. The samples were collected in clean polypropylene containers containing Cary Blair medium. These were transported to the Microbiology Department, Army Medical College, Rawalpindi within 1-2 hours. The samples were inoculated on Modified Preston (Oxoid) and Karmali media (Oxoid) beside other routine stool culture media. The cultures were incubated at 42 degrees C under microaerophilic conditions. The growth after 48 hours was provisionally identified by colonial morphology, oxidase test, Gram staining and motility. The organisms were identified to species level by hippurate hydrolysis, urease test, nitrate reduction, catalase test, H2S production and resistance to cephalothin.
Eighteen percent of samples yielded the growth of Campylobacter jejuni. Mean age of children with Campylobacter jejuni infection was 18 months with peak incidence from 12 to 21 months. Male female ratio was 1.7:1. All the children had loose motions. Seven out 18 (39%) had a combination of symptoms of loose motions, vomiting and pain abdomen. Those having fever with or without other complaints constituted 11 out of 18 (61.11%) i.e. more than 50% of all the children yielding C. jejuni had fever. About 90% of diarrhoeal stools had blood and fifty percent also had mucous. There was either history of chicken meat consumption or contact with cattle and pets in most of the cases and both in some of them.
Campylobacter jejuni is a frequent cause of diarrhoea/dysentery in children in our set up. In children it is often related to pets keeping and chicken meat consumption. In the remaining, untreated drinking water may be the source. Campylobacter jejuni frequently presents with blood and mucous in stools with sporadic cases presenting with watery diarrhoea.
确定2002年8月29日至2002年11月29日期间，拉瓦尔品第陆军医学院微生物科及军事医院中患腹泻/痢疾的儿童空肠弯曲菌感染率。
对拉瓦尔品第军事医院收治的100例12岁及以下腹泻/痢疾儿童的粪便样本进行研究。样本采集于装有 Cary Blair 培养基的清洁聚丙烯容器中。1 - 2小时内将其运送至拉瓦尔品第陆军医学院微生物科。除其他常规粪便培养基外，样本接种于改良普雷斯顿（Oxoid）和卡马利培养基（Oxoid）上。培养物在42℃微需氧条件下孵育。48小时后的生长情况通过菌落形态、氧化酶试验、革兰氏染色和动力进行初步鉴定。通过马尿酸盐水解、尿素酶试验、硝酸盐还原、过氧化氢酶试验、硫化氢产生及对头孢菌素的耐药性将菌株鉴定到种水平。
18%的样本培养出空肠弯曲菌。空肠弯曲菌感染儿童的平均年龄为18个月，发病高峰在12至21个月。男女比例为1.7:1。所有儿童均有腹泻。18例中有7例（39%）出现腹泻、呕吐和腹痛的症状组合。有发热（伴或不伴其他症状）的儿童占18例中的11例（61.11%），即所有培养出空肠弯曲菌的儿童中超过50%有发热症状。约90%的腹泻粪便带血，50%的粪便还有黏液。大多数病例有食用鸡肉史或与牛及宠物接触史，部分病例两者皆有。
在我们的研究中，空肠弯曲菌是儿童腹泻/痢疾的常见病因。在儿童中，其感染常与饲养宠物和食用鸡肉有关。其余病例中，未经处理的饮用水可能是感染源。空肠弯曲菌感染常表现为粪便带血和黏液，偶有病例表现为水样腹泻。
