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[黎巴嫩北部儿童腹泻中弯曲杆菌患病率的初步研究]

[Preliminary study on the prevalence of Campylobacter in childhood diarrhoea in north Lebanon].

作者信息

Dabboussi F, Alam S, Mallat H, Hlais S, Hamze M

机构信息

Laboratoire Microbiologie santé et environnement, Centre Azm pour la Recherche en Biotechnologie et ses Applications, Ecole doctorale des Sciences et de Technologie, Université Libanaise, Tripoli, Liban.

出版信息

East Mediterr Health J. 2012 Dec;18(12):1225-8.

Abstract

Campylobacter species are a major cause of human diarrhoeal disease worldwide. In Lebanon, the true prevalence of Campylobacter infections and the species distribution in childhood diarrhoea are not known. This study in 2010 investigated the prevalence of Campylobacter species and its possible etiologic role in childhood diarrhoea in north Lebanon. A total of 90 stool samples from children (aged 1 month to 10 years) presenting with diarrhoea were collected from 5 hospitals. A polymerase chain reaction technique (PCR) was used for each sample forthe amplification of all Campylobacter species followed by 5 PCR reactions for the amplification of C. jejuni, C. hyointestinalis, C. coli, C. fetus and C. upsaliensis. Of the 90 samples, 10 were positive for Campylobacterspecies (11.1%):1 for C.coli, 1 for C. jejuni, 2 for both C. jejuni and C. coli, and 6 could not be identified to the species level with the available primers. Campylobacter species is frequently associated with childhood diarrhoea in north Lebanon but Campylobacter infection may be significantly underdiagnosed because the search for Campylobacter is not part of the routine stool culture.

摘要

弯曲杆菌属是全球人类腹泻病的主要病因。在黎巴嫩,弯曲杆菌感染的实际流行情况以及儿童腹泻中的菌种分布尚不清楚。2010年的这项研究调查了黎巴嫩北部儿童腹泻中弯曲杆菌属的流行情况及其可能的病因作用。从5家医院收集了90份腹泻儿童(年龄1个月至10岁)的粪便样本。对每个样本使用聚合酶链反应技术(PCR)扩增所有弯曲杆菌属菌种,随后进行5次PCR反应以扩增空肠弯曲菌、猪肠弯曲菌、大肠弯曲菌、胎儿弯曲菌和乌普萨拉弯曲菌。在90个样本中,10个弯曲杆菌属菌种呈阳性(11.1%):1个为大肠弯曲菌阳性,1个为空肠弯曲菌阳性,2个为空肠弯曲菌和大肠弯曲菌均阳性,6个用现有引物无法鉴定到菌种水平。在黎巴嫩北部,弯曲杆菌属菌种常与儿童腹泻相关,但由于常规粪便培养中不包括对弯曲杆菌的检测,弯曲杆菌感染可能被严重漏诊。

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