Igout J, Fretigny M, Vasse M, Callat M P, Silva M, Willemont L, Gelle M, Lenormand B
Laboratoire d'hématologie, CHU Charles Nicolle, Rouen, France.
Clin Lab Haematol. 2004 Feb;26(1):1-7. doi: 10.1111/j.0141-9854.2003.00577.x.
The Coulter LH 750 is a new haematology analyser with several new features: a count of nucleated red blood cells (NRBCs), automated WBC correction in presence of a flag indicating a cellular interference and a lower incidence of platelet or WBC interference flags when compared with the GEN.S, our current instrument. We had three main goals in our study: evaluating the LH 750 WBC counts when a GEN.S flag suggests a risk of WBC interference, ascertaining whether the platelet counts not flagged by the LH 750 were accurately assessed in samples flagged by the GEN.S and evaluating the NRBC assay provided by the LH 750. Flow cytometry, using CD45 and CD41, respectively for WBC and platelet labelling, was used as a reference method to assess the accuracy of the LH 750 counts. NRBC were identified by double labelling with propidium iodide (PI) and CD45, NRBCs being CD45-/PI+. A significant relationship was found between LH 750 and flow cytometric WBC counts, whether a WBC correction was made by the LH 750 (r = 0.9809, n = 54) or not (r = 0.9901, n = 23). A highly significant relationship was observed for platelets not only in the range from 0 to 450 x 10(9)/l (r = 0.981, n = 108) but also in cases of thrombocytopenia (range: 0-80 x 10(9)/l; r = 0.956, n = 51). In samples with NRBCs, the NRBC percentages given by the LH 750 and by flow cytometry were highly correlated (r = 0.977, n = 60) and WBC counts were accurate. In conclusion, the reduction in flagging by the LH 750, the accuracy of the results, and the availability of a NRBC count, constitute major advantages.
库尔特LH 750是一款新型血液分析仪，具有多项新特性：可计数有核红细胞（NRBC），在出现提示细胞干扰的标记时能自动校正白细胞计数，与我们当前使用的GEN.S仪器相比，血小板或白细胞干扰标记的发生率更低。我们的研究有三个主要目标：当GEN.S标记提示存在白细胞干扰风险时，评估LH 750的白细胞计数；确定LH 750未标记的样本中，GEN.S标记的样本的血小板计数是否得到准确评估；评估LH 750提供的NRBC检测方法。分别使用CD45和CD41标记白细胞和血小板的流式细胞术被用作评估LH 750计数准确性的参考方法。通过碘化丙啶（PI）和CD45双重标记鉴定NRBC，NRBC为CD45 - /PI + 。无论LH 750是否进行白细胞校正，LH 750与流式细胞术白细胞计数之间均存在显著相关性（r = 0.9809，n = 54）或（r = 0.9901，n = 23）。不仅在0至450×10⁹/L范围内血小板存在高度显著相关性（r = 0.981，n = 108），在血小板减少症病例中（范围：0 - 80×10⁹/L；r = 0.956，n = 51）也是如此。在含有NRBC的样本中，LH 750和流式细胞术给出的NRBC百分比高度相关（r = 0.977，n = 60），且白细胞计数准确。总之，LH 750标记减少、结果准确性以及NRBC计数的可用性构成了主要优势。
