秀丽隐杆线虫中系统性RNA干扰所需的基因。

Genes required for systemic RNA interference in Caenorhabditis elegans.

作者信息

Tijsterman Marcel, May Robin C, Simmer Femke, Okihara Kristy L, Plasterk Ronald H A

机构信息

Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.

出版信息

Curr Biol. 2004 Jan 20;14(2):111-6. doi: 10.1016/j.cub.2003.12.029.

DOI:10.1016/j.cub.2003.12.029

PMID:14738731

Abstract

RNA interference (RNAi) in the nematode worm, Caenorhabditis elegans, occurs systemically. Double-stranded RNA (dsRNA) provided in the diet can be absorbed from the gut lumen and distributed throughout the body, triggering RNAi in tissues that are not exposed to the initial dsRNA trigger. This is in marked contrast to other animals, in which RNAi does not spread from targeted tissues to neighboring cells. Here, we report the characterization of mutants defective in the systemic aspect of RNAi, but not in the core RNAi process itself. Analysis of these mutants suggests that dsRNA uptake is a specific process involving several unique proteins.

摘要

线虫秀丽隐杆线虫中的RNA干扰（RNAi）是全身性发生的。饮食中提供的双链RNA（dsRNA）可从肠腔吸收并分布到全身，在未接触初始dsRNA触发物的组织中引发RNAi。这与其他动物形成鲜明对比，在其他动物中RNAi不会从靶向组织扩散到相邻细胞。在这里，我们报告了在RNAi全身性方面存在缺陷但在核心RNAi过程本身不存在缺陷的突变体的特征。对这些突变体的分析表明，dsRNA摄取是一个涉及几种独特蛋白质的特定过程。