Schmalenberg K E, Buettner H M, Uhrich K E
Department of Chemistry and Chemical Biology, 610 Taylor Road, Rutgers University, Piscataway, NJ 08854, USA.
Biomaterials. 2004 May;25(10):1851-7. doi: 10.1016/j.biomaterials.2003.08.048.
This paper describes a method for microcontact printing protein solutions onto polymer substrates temporarily activated by oxygen plasma. Following plasma treatment, poly(dimethyl siloxane) (PDMS) stamps were coated with an aqueous laminin solution then placed in direct contact with plasma-treated poly(methyl methacrylate) (PMMA) substrates. This process resulted in well defined laminin stripes on the PMMA surface when printing was performed within 45min of the plasma treatment. Axonal outgrowth from embryonic chick dorsal root ganglia (DRG) was largely confined to the stamped pattern, while over 90% of primary rat Schwann cells adhered to the protein stamped areas on the PMMA substrates. Oxygen-plasma treatment of the PMMA surface was necessary to deposit proteins that direct axonal outgrowth from chick DRG and Schwann cell adherence.
本文描述了一种将蛋白质溶液微接触印刷到由氧等离子体临时活化的聚合物基底上的方法。等离子体处理后，将聚二甲基硅氧烷（PDMS）印章涂上层粘连蛋白水溶液，然后与经等离子体处理的聚甲基丙烯酸甲酯（PMMA）基底直接接触。当在等离子体处理后的45分钟内进行印刷时，该过程在PMMA表面产生了界限清晰的层粘连蛋白条纹。胚胎鸡背根神经节（DRG）的轴突生长主要局限于印刷图案，而超过90%的原代大鼠雪旺细胞粘附在PMMA基底上的蛋白质印刷区域。对PMMA表面进行氧等离子体处理对于沉积引导鸡DRG轴突生长和雪旺细胞粘附的蛋白质是必要的。
