Lin Wen-Ching, Liu Ting-Yu, Yang Ming-Chien
Department of Polymer Engineering, National Taiwan University of Science and Technology, 106, ROC, Taipei, Taiwan.
Biomaterials. 2004 May;25(10):1947-57. doi: 10.1016/j.biomaterials.2003.08.027.
Chitosan (CS)/heparin (HEP) polyelectrolyte complex (PEC) was covalently immobilized onto the surface of polyacrylonitrile (PAN) membrane. The effect of surface modification on the protein adsorption and platelet adhesion, metabolites permeation and anticoagulation activity of the resulting membrane was investigated. Surface characterization such as water contact angle, and X-ray photoelectron spectroscope were performed. The immobilization of PEC caused the water contact angle to reduce, thereby indicating the increase in the hydrophilicity. Protein adsorption, platelet adhesion, and thrombus formation were all reduced by the immobilization of HEP. Anticoagulant activity was evaluated with activated partial thrombin time (APTT), prothrombin time (PT), fibrinogen time, and thrombin time (TT). The results revealed that PEC-immobilizing membrane can improve antithrombogenicity of PAN membrane. In addition, the PEC-immobilized membranes can suppress the proliferation of Pseudomonas aeruginosa. In vitro cytotoxicity test showed leachable substance released was below cytotoxic level. The pure water permeability results show little variation due to PEC-immobilization. Thus PEC-immobilization can endow the PAN membrane hemocompatibility and antibacterial activity while retaining the original permeability.
壳聚糖（CS）/肝素（HEP）聚电解质复合物（PEC）被共价固定在聚丙烯腈（PAN）膜表面。研究了表面改性对所得膜的蛋白质吸附、血小板黏附、代谢物渗透及抗凝活性的影响。进行了诸如水接触角和X射线光电子能谱等表面表征。PEC的固定导致水接触角减小，从而表明亲水性增加。HEP的固定降低了蛋白质吸附、血小板黏附和血栓形成。用活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、纤维蛋白原时间和凝血酶时间（TT）评估抗凝活性。结果表明，固定PEC的膜可改善PAN膜的抗血栓形成能力。此外，固定PEC的膜可抑制铜绿假单胞菌的增殖。体外细胞毒性试验表明，释放的可浸出物质低于细胞毒性水平。纯水渗透性结果显示，由于PEC的固定，变化很小。因此，PEC的固定可赋予PAN膜血液相容性和抗菌活性，同时保留其原始渗透性。
