Lin Wen-Ching, Tseng Chien-Hao, Yang Ming-Chien
Department of Polymer Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, 106, ROC.
Macromol Biosci. 2005 Oct 20;5(10):1013-21. doi: 10.1002/mabi.200500077.
The surface of a thermoplastic polyurethane (TPU) membrane was treated with low temperature plasma (LTP) and was then grafted with poly(acrylic acid) (PAA), followed by the grafting of water-soluble chitosan (WSC) and heparin (HEP). The surface was characterized with static contact-angle and X-ray photoelectron spectroscopy (XPS). The results showed that the surface densities of peroxides and PAA reached a maximum when treated with LTP for 90 s. A higher pH of the reacting solution led to higher graft densities of WSC and HEP. After WSC and HEP grafting, the hydrophilicity of the TPU membrane was increased. The adsorption of proteins on HEP-grafted TPU membranes was effectively curtailed. In addition, HEP grafting also reduced platelet adhesion, elevated thrombin inactivation, and prolonged the blood coagulation time. According to the L929 fibroblast cell growth inhibition index, the HEP-grafted TPU membranes exhibited non-cytotoxicity. Overall results demonstrated that the HEP immobilization could not only improve the hydrophilicity but also the hemocompatibility of the TPU membrane, while maintaining the ascendant biocompatibility.
对热塑性聚氨酯（TPU）膜表面进行低温等离子体（LTP）处理，然后接枝聚丙烯酸（PAA），接着接枝水溶性壳聚糖（WSC）和肝素（HEP）。采用静态接触角和X射线光电子能谱（XPS）对表面进行表征。结果表明，LTP处理90 s时，过氧化物和PAA的表面密度达到最大值。反应溶液的pH值越高，WSC和HEP的接枝密度越高。接枝WSC和HEP后，TPU膜的亲水性增加。HEP接枝的TPU膜上蛋白质的吸附被有效抑制。此外，HEP接枝还减少了血小板黏附，提高了凝血酶失活率，并延长了凝血时间。根据L929成纤维细胞生长抑制指数，HEP接枝的TPU膜表现出无细胞毒性。总体结果表明，HEP固定化不仅可以提高TPU膜的亲水性，还可以提高其血液相容性，同时保持良好的生物相容性。
