Li Xiaoxu, Taylor John Stephen
Department of Chemistry, Washington University in St Louis, St. Louis, MO 63130, USA.
Bioorg Med Chem. 2004 Feb 1;12(3):545-52. doi: 10.1016/j.bmc.2003.11.016.
To study ester prodrug stability properties in living cells we have conjugated fluorogenic esters to the cell membrane permeable peptide Arg9. The desired conjugates are prepared by coupling N-maleoyl amino acid esters of monoalkylated fluoresceins or fluorescein to TyrArg9Cys. The photophysical properties of the monoalkylated fluorescein derivatives are described.
为了研究酯前药在活细胞中的稳定性，我们将荧光酯与细胞膜可渗透肽Arg9偶联。所需的偶联物通过将单烷基化荧光素或荧光素的N-马来酰氨基酸酯与TyrArg9Cys偶联来制备。描述了单烷基化荧光素衍生物的光物理性质。
