Ji Shengxiang, Zhu Zhengxi, Hoye Thomas R, Macosko Christopher W
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Macromol Chem Phys. 2009 May 22;210(10):823. doi: 10.1002/macp.200900025.
Carboxylic acid terminated poly(ε-caprolactone)s (PCL-COOHs) with narrow polydispersity were synthesized and coupled with poly(ethylene glycol) (HO-PEG-OH) to afford PCL-PEG-OH copolymers. The hydroxyl groups in the PCL-PEG-OHs were then converted to maleimide groups to afford maleimide terminated PCL-PEG-MALs that contained 70-90% maleimide functionality. Nanoparticles with maleimide functionality on their surfaces were prepared by impingement mixing. Particle sizes and size distributions were determined by dynamic light scattering. Conjugation of reduced glutathione with model maleimides and two MAL-functional nanoparticles was also demonstrated. The amount of accessible maleimide on the particle surface was measured using Ellman's reagent to range between ~51-67%.
合成了具有窄多分散性的羧酸封端聚（ε-己内酯）（PCL-COOHs），并将其与聚乙二醇（HO-PEG-OH）偶联，得到PCL-PEG-OH共聚物。然后将PCL-PEG-OH中的羟基转化为马来酰亚胺基团，得到含有70-90%马来酰亚胺官能度的马来酰亚胺封端的PCL-PEG-MALs。通过冲击混合制备了表面具有马来酰亚胺官能度的纳米颗粒。通过动态光散射测定颗粒大小和尺寸分布。还证明了还原型谷胱甘肽与模型马来酰亚胺和两种MAL官能化纳米颗粒的共轭。使用埃尔曼试剂测量颗粒表面可及马来酰亚胺的量，范围在约51-67%之间。
