Matsuo Hirotami, Chevallier Julien, Mayran Nathalie, Le Blanc Isabelle, Ferguson Charles, Fauré Julien, Blanc Nathalie Sartori, Matile Stefan, Dubochet Jacques, Sadoul Rémy, Parton Robert G, Vilbois Francis, Gruenberg Jean
Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
Science. 2004 Jan 23;303(5657):531-4. doi: 10.1126/science.1092425.
What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.
控制真核细胞中亚细胞器组装的组件有哪些？尽管膜显然会被胞质因子扭曲，但对于控制细胞器膜生物发生、形状和组织的内在机制却知之甚少。在这里，我们发现非常规磷脂溶血双磷脂酸（LBPA）可诱导形成多囊泡脂质体，其类似于在体内发现该脂质的部位存在的多囊泡内体。这个过程依赖于体内跨内体膜存在的相同pH梯度，并由Alix选择性控制。反过来，Alix在体内调节含LBPA的内体的组织。
