Chang Sung-Eun, Kim Kyoung-Jin, Ro Kyoung-Hyun, Lim Young-Jin, Choi Jee-Ho, Moon Kee-Chan, Sung Kyung-Jeh
Department of Dermatology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
J Dermatol. 2004 Jan;31(1):1-5. doi: 10.1111/j.1346-8138.2004.tb00495.x.
Keloids are often resistant to treatment, causing much suffering to the patient. Our previous work found that ceramide (Cer) inhibits growth of fibroblasts via apoptosis. However, when compared to normal fibroblasts (NFs), which are quiescent, keloid fibroblasts (KFs) rapidly proliferate and are reported to be resistant to apoptosis via Cer. Sphingosine (Sph) is a metabolite product of ceramide that has some different biochemical properties. Thereofore, we investigated the cytotoxic effects of Sph on cultured fibroblasts from keloid lesions and normal skin in order to evaluate the possibility of using Sph in the treatment of keloid. We used the lactic dehydrogenase (LDH) method, MTT method, and propidium iodide (PI) method. Sph had cytotoxic effects via apoptosis on both the KFs and NFs. Our results indicate that Sph may be applicable to the future treatment of keloid.
瘢痕疙瘩通常对治疗有抗性，给患者带来很大痛苦。我们之前的研究发现，神经酰胺（Cer）通过凋亡抑制成纤维细胞生长。然而，与静止的正常成纤维细胞（NFs）相比，瘢痕疙瘩成纤维细胞（KFs）迅速增殖，并且据报道对神经酰胺介导的凋亡具有抗性。鞘氨醇（Sph）是神经酰胺的代谢产物，具有一些不同的生化特性。因此，我们研究了鞘氨醇对瘢痕疙瘩病变和正常皮肤培养的成纤维细胞的细胞毒性作用，以评估使用鞘氨醇治疗瘢痕疙瘩的可能性。我们使用了乳酸脱氢酶（LDH）法、MTT法和碘化丙啶（PI）法。鞘氨醇通过凋亡对瘢痕疙瘩成纤维细胞和正常成纤维细胞均具有细胞毒性作用。我们的结果表明，鞘氨醇可能适用于未来瘢痕疙瘩的治疗。
