Champion A, Kreis M, Mockaitis K, Picaud A, Henry Y
Institut de Biotechnologie des Plantes, Laboratoire de Biologie du Développement des Plantes, Bâtiment 630, UMR CNRS/UPS 8618, Université de Paris-Sud, 91405, Orsay Cedex, France.
Funct Integr Genomics. 2004 Jul;4(3):163-87. doi: 10.1007/s10142-003-0096-4. Epub 2004 Jan 22.
Arabidopsis thaliana is used as a favourite experimental organism for many aspects of plant biology. We capitalized on the recently available Arabidopsis genome sequence and predicted proteome, to draw up a genome-scale protein serine/threonine kinase (PSTK) inventory. The PSTKs represent about 4% of the A. thaliana proteome. In this study, we provide a description of the content and diversity of the non-receptor PSTKs. These kinases have crucial functions in sensing, mediating and coordinating cellular responses to an extensive range of stimuli. A total of 369 predicted non receptor PSTKs were detailed: the Raf superfamily, the CMGC, CaMK, AGC and STE families, as well as a few small clades and orphan sequences. An extensive relationship analysis of these kinases allows us to classify the proteins in superfamilies, families, sub-families and groups. The classification provides a better knowledge of the characteristics shared by the different clades. We focused on the MAP kinase module elements, with particular attention to their docking sites for protein-protein interaction and their biological function. The large number of A. thaliana genes encoding kinases might have been achieved through successive rounds of gene and genome duplications. The evolution towards an increasing gene number suggests that functional redundancy plays an important role in plant genetic robustness.
拟南芥被用作植物生物学许多方面研究的常用实验生物。我们利用最近可得的拟南芥基因组序列和预测的蛋白质组，编制了一份基因组规模的蛋白质丝氨酸/苏氨酸激酶（PSTK）清单。PSTK约占拟南芥蛋白质组的4%。在本研究中，我们描述了非受体PSTK的组成和多样性。这些激酶在感知、介导和协调细胞对广泛刺激的反应中具有关键作用。共详细分析了369个预测的非受体PSTK：Raf超家族、CMGC、CaMK、AGC和STE家族，以及一些小分支和孤儿序列。对这些激酶进行广泛的关系分析，使我们能够将蛋白质分类为超家族、家族、亚家族和组。这种分类有助于更好地了解不同分支共有的特征。我们重点关注丝裂原活化蛋白激酶（MAPK）模块元件，特别关注它们用于蛋白质-蛋白质相互作用的对接位点及其生物学功能。拟南芥中编码激酶的大量基因可能是通过连续的基因和基因组复制产生的。基因数量不断增加的进化表明，功能冗余在植物遗传稳健性中起着重要作用。
