[Study on effects of interleukin-1 and their inhibitors for proliferation and hyaluronic acid synthesis of cultured human orbital fibroblasts].

PURPOSE

To observe the proliferation and synthesis of hyaluronic acid (HA) of cultured human orbital fibroblasts (OFs) affected by interleukin-1 (IL-1), and their inhibitors interleukin-1 receptor antagonist(IL-1Ra).

METHODS

OFs from 5 patients with thyroid-associated ophthalmopathy (TAO) and 4 normal subjects were cultured in vitro. IL-1, IL-1Ra with different concentrations were put into media respectively, and IL-1(500 U/ml), IL-1Ra(5, 50 ng/ml) were put into media together. The proliferation and HA production of OFs were measured by MTT and radioimmunological technique.

RESULTS

IL-1(550,500 U/ml) can significantly increase the proliferation of OFs and synthesis of HA (P < 0.05), IL-1 (500 U/ml) stimulate more proliferation of OFs and HA production of OFs from TAO patients than that of OFs from normal subjects. While IL-1Ra alone had no inhibitive effects at any concentrations. IL1Ra (5.50 ng/ml) can block the proliferation and HA production of OFs when IL-1 with IL-1Ra incubated together.

CONCLUSION

IL-1 can stimulate the proliferation and HA synthesis of OFs. IL-1Ra is an effective inhibitor of proliferation and synthesis of OFs induced by IL-1. IL-1Ra may be useful in the prevention or treatment of TAO.