随着离子强度增加角膜胶原纤维的排列

The ordering of corneal collagen fibrils with increasing ionic strength.

作者信息

Regini J W, Elliott G F, Hodson S A

机构信息

The Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff CF10 3NB, UK.

出版信息

J Mol Biol. 2004 Feb 6;336(1):179-86. doi: 10.1016/j.jmb.2003.12.001.

DOI:10.1016/j.jmb.2003.12.001

PMID:14741213

Abstract

The fixed stromal charge of bovine corneas, osmotically clamped at physiological hydration, was altered by regulating the amount of chloride ions bound to the matrix. We measured the local fibrillar collagen order using X-ray diffraction methods. As the bound anions increased up to physiological values, the local fibrillar order increased to an optimal value. The coherence distance (t) approximately doubles to a maximum value (409 nm) from 10 mM NaCl to 154 mM NaCl. This then slowly decreased as the bathing solution increased to 1000 mM. In contrast the diameter of the collagen fibrils were minimal at physiological NaCl.

摘要

通过调节与基质结合的氯离子数量，改变了在生理水合状态下进行渗透钳制的牛角膜的固定基质电荷。我们使用X射线衍射方法测量了局部纤维状胶原的有序度。随着结合阴离子增加至生理值，局部纤维状有序度增加至最佳值。相干长度（t）从10 mM氯化钠到154 mM氯化钠时大约翻倍至最大值（409 nm）。然后随着浴液浓度增加至1000 mM，相干长度缓慢下降。相比之下，胶原纤维的直径在生理氯化钠浓度下最小。