Leonard D W, Meek K M
Open University, Oxford Research Unit, United Kingdom.
Biophys J. 1997 Mar;72(3):1382-7. doi: 10.1016/S0006-3495(97)78784-8.
Ultrastructural data from x-ray diffraction studies of the cornea were used to estimate the refractive indices of the collagen fibrils and extrafibrillar material of human, ox, trout, and rabbit corneas. X-ray diffraction measurements of the size and spacing of the collagen fibrils and the separation between the constituent molecules of the fibrils were taken from a previous species study. The tissue volume fractions occupied by the stromal components were estimated and their refractive indices were calculated using the Gladstone-Dale law of mixtures. For the fibrils and extrafibrillar material, the refractive indices in the human cornea were 1.411 and 1.365; for the ox 1.413 and 1.357; for the rabbit 1.416 and 1.357; and for the trout 1.418 and 1.364, respectively. An alternative estimate based on the physical properties and chemical composition of bovine cornea, accounting for interfibrillar type VI collagen and cellular water, produced similar estimates of 1.416 and 1.356 for the fibrils and extrafibrillar material, respectively.
利用来自角膜X射线衍射研究的超微结构数据，估算人、牛、鳟鱼和兔角膜中胶原纤维和纤维外物质的折射率。胶原纤维的大小和间距以及纤维组成分子间的间距的X射线衍射测量数据取自先前的物种研究。估算了基质成分所占的组织体积分数，并使用格拉德斯通-戴尔混合定律计算其折射率。对于纤维和纤维外物质，人角膜中的折射率分别为1.411和1.365；牛角膜中为1.413和1.357；兔角膜中为1.416和1.357；鳟鱼角膜中为1.418和1.364。基于牛角膜的物理性质和化学成分，考虑到纤维间VI型胶原和细胞内水，对纤维和纤维外物质分别得出了类似的估算值，即1.416和1.356。
