Wemhoff G A, Rabbany S Y, Kusterbeck A W, Ogert R A, Bredehorst R, Ligler F S
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375.
J Immunol Methods. 1992 Dec 8;156(2):223-30. doi: 10.1016/0022-1759(92)90029-s.
We have developed the theoretical framework for a displacement immunoassay conducted in flow under nonequilibrium conditions. Using a repetitive displacement technique, we determined the displacement rate and apparent dissociation rate constant at different flow rates. Our data suggest that the kinetics are best described by a first-order function. The displacement efficiency, the displacement rate, and therefore the apparent dissociation rate constant were calculated and demonstrated to be flow rate dependent. The theoretical framework developed in this study was successful in predicting the behavior of antigen displacement in flow.
我们已经开发出了一种在非平衡条件下于流动中进行的置换免疫分析的理论框架。采用重复置换技术，我们测定了不同流速下的置换速率和表观解离速率常数。我们的数据表明，动力学最好用一阶函数来描述。计算了置换效率、置换速率以及因此的表观解离速率常数，并证明它们与流速有关。本研究中开发的理论框架成功地预测了流动中抗原置换的行为。
