Kaur Parminder, Bansal M P
Department of Biophysics, Panjab University, Chandigarh-160014, India.
Biol Trace Elem Res. 2004 Jan;97(1):83-93. doi: 10.1385/BTER:97:1:83.
The present study was carried out to evaluate the effect of selenium (Se)-induced oxidative stress on the oxidation reduction system and the fertility status of male mice. Different levels of Se, a potent antioxidant, were fed in three separate groups for 8 wk to create the different oxidative stress in mice. A significant decrease in the glutathione peroxidase (GSH-Px) in both liver and testis was observed in the Se-deficient (0.02 ppm) group I, whereas enzyme levels in the Se-excess (1 ppm) group were comparable to the Se-adequate (0.2 ppm) group. Glutathione-S-transferase activity was enhanced in group I in comparison to group II; however, no change was seen in group III. The glutathione reductase and superoxide dismutase activities were decreased in the Se-deficient group, whereas the enzyme levels were significantly increased in the Se-excess group. The fertility status of the animals studied in terms of percentage fertility and litter size showed a significant decrease in the reproductive ability of male mice in group I when compared to group II. No changes in the fertility status of animals were observed in group III. Thus, the data clearly indicate the effect of oxidative stress generated by feeding various Se levels on the oxidation reduction system and, consequently, its effect on the reproductive ability of male mice.
本研究旨在评估硒（Se）诱导的氧化应激对雄性小鼠氧化还原系统和生育状况的影响。将不同水平的强效抗氧化剂硒分别喂给三个独立的组，持续8周，以在小鼠体内产生不同程度的氧化应激。在缺硒（0.02 ppm）的I组中，肝脏和睾丸中的谷胱甘肽过氧化物酶（GSH-Px）显著降低，而高硒（1 ppm）组的酶水平与适硒（0.2 ppm）组相当。与II组相比，I组的谷胱甘肽-S-转移酶活性增强；然而，III组未见变化。缺硒组的谷胱甘肽还原酶和超氧化物歧化酶活性降低，而高硒组的酶水平显著升高。从生育百分比和窝仔数方面研究动物的生育状况，结果显示，与II组相比，I组雄性小鼠的生殖能力显著下降。III组动物的生育状况未观察到变化。因此，数据清楚地表明了通过喂食不同水平的硒所产生的氧化应激对氧化还原系统的影响，进而对雄性小鼠生殖能力的影响。
