Macve Joanna C, McKenzie Roddie C, Norval Mary
Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh, UKEH8 9AG.
Photochem Photobiol Sci. 2004 Jan;3(1):91-5. doi: 10.1039/b306564b. Epub 2003 Aug 28.
Immune suppression following UVB irradiation is partly attributed to the effects of the exposure on antigen-presenting cells. Following a single UVB irradiation, there is a decrease in epidermal Langerhans cell numbers; this is accompanied by an increase in the number of dendritic cells (DC) in lymph nodes draining the irradiated site. We investigated whether a similar effect occurred following multiple UVB exposures. Mice were irradiated on their ears and shaved dorsal skin twice a week for 3 weeks. After the final exposure, the number of ATPase(+) Langerhans cells in epidermal sheets prepared from the ears was found to be decreased by 33% compared to unirradiated controls. The number of DC in the draining lymph nodes (DLN) did not increase as might have been expected; rather, a significant decrease of approximately 30% in DC numbers in the DLN of UVB-irradiated mice compared with unirradiated controls occurred. This decrease in antigen-presenting cells in both the epidermis and the DLN may be an important contributing factor to the immune suppression that follows multiple UVB exposures.
紫外线B（UVB）照射后的免疫抑制部分归因于该照射对抗原呈递细胞的影响。单次UVB照射后，表皮朗格汉斯细胞数量减少；与此同时，受照射部位引流淋巴结中的树突状细胞（DC）数量增加。我们研究了多次UVB照射后是否会出现类似效应。每周两次对小鼠耳部和背部剃毛皮肤进行照射，持续3周。末次照射后，发现从耳部制备的表皮片中ATP酶（+）朗格汉斯细胞数量与未照射对照组相比减少了33%。引流淋巴结（DLN）中的DC数量并未如预期那样增加；相反，与未照射对照组相比，UVB照射小鼠DLN中的DC数量显著减少了约30%。表皮和DLN中抗原呈递细胞的这种减少可能是多次UVB照射后免疫抑制的一个重要促成因素。
