Boissinot Stéphane, Roos Christian, Furano Anthony V
Section on Genomic Structure and Function, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
J Mol Evol. 2004 Jan;58(1):122-30. doi: 10.1007/s00239-003-2539-x.
LINE-1 (L1) elements constitute the major family of retrotransposons in mammalian genomes. Here we report the first investigation of L1 evolution in New World monkeys (NWM). Two regions of the second open-reading frame were analyzed by two methods in three NWM species, the squirrel monkey (Saimiri sciureus), the tamarin (Saguinus oedipus), and the spider monkey (Ateles paniscus). Since these three species diverged, L1 has amplified in the Saimiri and Saguinus lineages but L1 activity seems to have been strongly reduced in the Ateles lineage. In addition, the active L1 lineage has evolved rapidly in Saimiri and Saguinus, generating species-specific subfamilies. In contrast, we found no evidence for a species-specific subfamily in Ateles, a result consistent with the low L1 activity in this species for the last approximately 25 My.
LINE-1(L1)元件是哺乳动物基因组中逆转座子的主要家族。在此,我们报告了对新大陆猴(NWM)中L1进化的首次研究。在三种新大陆猴物种——松鼠猴(Saimiri sciureus)、绢毛猴(Saguinus oedipus)和蜘蛛猴(Ateles paniscus)中,通过两种方法对第二个开放阅读框的两个区域进行了分析。自从这三个物种分化以来,L1在松鼠猴和绢毛猴谱系中发生了扩增,但在蜘蛛猴谱系中L1活性似乎已大幅降低。此外,活跃的L1谱系在松鼠猴和绢毛猴中快速进化,产生了物种特异性亚家族。相比之下,我们没有发现蜘蛛猴存在物种特异性亚家族的证据,这一结果与该物种在过去约2500万年中较低的L1活性一致。