Bernstein Hans-Gert, Keilhoff Gerburg, Bukowska Alicja, Ziegeler Anke, Funke Sieglinde, Dobrowolny Henrik, Kanakis Dimitrios, Bogerts Bernhard, Lendeckel Uwe
Department of Psychiatry, University of Magdeburg, Magdeburg, Germany.
J Neurosci Res. 2004 Feb 1;75(3):353-60. doi: 10.1002/jnr.10858.
ADAM12 is a member of the large family of multidomain metalloprotease-disintegrins which possess cell-binding and metalloprotease properties. Typically, ADAM12 is expressed in mesenchymal cells, developing and regenerating heart and skeletal muscle, bone as well as in certain tumours. This report shows by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry that the protease ADAM12 is detectable in human and rat brain tissue as well as in cultured cells derived from rat brain. With the exception of a very few immunopositive pyramidal neurons in the developing rat brain, the cellular localization of ADAM12 was exclusively confined to oligodendroglial cells. Thus, ADAM12 may be regarded a new suitable marker for this cell type.
ADAM12是多结构域金属蛋白酶-解整合素大家族的成员，具有细胞结合和金属蛋白酶特性。通常，ADAM12在间充质细胞、发育和再生中的心脏与骨骼肌、骨骼以及某些肿瘤中表达。本报告通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学显示，蛋白酶ADAM12在人和大鼠脑组织以及源自大鼠脑的培养细胞中均可检测到。除了发育中的大鼠脑中极少数免疫阳性的锥体神经元外，ADAM12的细胞定位仅局限于少突胶质细胞。因此，ADAM12可被视为这种细胞类型的一种新的合适标志物。
