Tian B L, Wen J M, Zhang M, Xie D, Xu R B, Luo C J
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, People's Republic of China.
Mol Pathol. 2002 Dec;55(6):394-7. doi: 10.1136/mp.55.6.394.
To examine the expression of ADAM12 (meltrin alpha), a member of the disintegrin and metalloprotease (ADAM) family, in human giant cell tumours of the bone, skeletal muscle tissue from human embryos, and human adult skeletal muscle tissue.
ADAM12 mRNA was detected by reverse transcription polymerase chain reaction and in situ hybridisation.
ADAM12 mRNA was detected in 14 of the 20 giant cell tumours of bone and in three of the six tumour cell cultures. The expression of ADAM12 in cells cultured from the tumour was linked to the presence of multinucleated giant cells. ADAM12 mRNA could not be detected in the five adult skeletal muscle tissue samples, although it was found in the two embryonic skeletal muscle tissue samples. ADAM12 mRNA was localised to the cytoplasm of multinucleated giant cells and some mononuclear stromal cells.
These results indicate that multinucleated giant cells are formed by the cell fusion of mononuclear stromal cells in giant cell tumours of bone and that ADAM12 is involved in the cell fusion process.
检测去整合素和金属蛋白酶（ADAM）家族成员ADAM12（黑素瘤α）在人骨巨细胞瘤、人胚胎骨骼肌组织及成人骨骼肌组织中的表达。
采用逆转录聚合酶链反应和原位杂交检测ADAM12 mRNA。
在20例骨巨细胞瘤中的14例以及6个肿瘤细胞培养物中的3个中检测到ADAM12 mRNA。肿瘤培养细胞中ADAM12的表达与多核巨细胞的存在有关。在5份成人骨骼肌组织样本中未检测到ADAM12 mRNA，而在2份胚胎骨骼肌组织样本中发现了该mRNA。ADAM12 mRNA定位于多核巨细胞和一些单核基质细胞的细胞质中。
这些结果表明，骨巨细胞瘤中的多核巨细胞是由单核基质细胞融合形成的，且ADAM12参与了细胞融合过程。
