Apte S P, Turco S J
School of Pharmacy, Temple University, Philadelphia, PA.
J Parenter Sci Technol. 1992 Nov-Dec;46(6):233-8.
A novel method for characterization of size distributions of parenteral fluorocarbon emulsions is described. Prepared emulsions were centrifuged to sediment droplets above predetermined diameters out of a known supernatant sample volume using the Bostok-Stoke's equation. Centrifugation times may be calculated using centrifuge parameters and physical properties of the fluorocarbon oil phase and the dispersion medium. The fluorocarbon content of the supernatant sample volume at successive centrifugation times was determined both by densitometry and by Gas Chromatography. A close correlation was found between the two methods. The density data was processed and converted into a volume distribution histogram by means of a program written in BASIC. The speed, simplicity of use, non reliance on costly equipment and good correlation to absolute particle counting methods makes the density method suitable for submicron size characterization.
描述了一种表征肠胃外氟碳乳液粒径分布的新方法。使用博斯托克-斯托克斯方程，将制备好的乳液离心，使直径大于预定值的液滴从已知的上清液样本体积中沉淀出来。离心时间可根据离心机参数以及氟碳油相和分散介质的物理性质来计算。通过密度测定法和气相色谱法测定连续离心时间下上清液样本体积中的氟碳含量。发现这两种方法之间有密切的相关性。利用用BASIC语言编写的程序对密度数据进行处理并转换为体积分布直方图。密度法具有速度快、使用简单、不依赖昂贵设备以及与绝对颗粒计数方法相关性好的特点，适用于亚微米尺寸表征。
