Lee Mao-Jung, Lambert Joshua D, Prabhu Saileta, Meng Xiaofeng, Lu Hong, Maliakal Pius, Ho Chi-Tang, Yang Chung S
Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Cancer Epidemiol Biomarkers Prev. 2004 Jan;13(1):132-7. doi: 10.1158/1055-9965.epi-03-0040.
Catechins and theaflavins, polyphenolic compounds derived from tea (Camellia sinensis, fam. Theaceae), have been reported to have a wide range of biological activities including prevention of tooth decay and oral cancer. The present study was undertaken to determine the usefulness of green tea leaves and black tea extract for the delivery of catechins and theaflavins to the oral cavity. After holding either green tea leaves (2 g) or brewed black tea (2 g of black tea leaves in 100 ml) in the mouth for 2-5 min and thoroughly rinsing the mouth, high concentrations of catechins (C(max) = 131.0-2.2 micro M) and theaflavins (C(max) = 1.8-0.6 micro M) were observed in saliva in the 1st hour. Whereas there was significant interindividual variation in the peak levels of catechins and theaflavins, the overall kinetic profile was similar, with t(1/2) = 25-44 min and 49-76 min for catechins and theaflavins, respectively (average coefficient of variation in t(1/2) was 23.4%). In addition to the parent catechin and theaflavin peaks, five unidentified peaks were also observed in saliva after black tea treatment. Hydrolysis of theaflavin gallates, apparently by salivary esterases, was observed in vitro and in vivo. These results indicate that tea leaves can be used as a convenient, slow-release source of catechins and theaflavins and provide information for the possible use of tea in the prevention of oral cancer and dental caries.
儿茶素和茶黄素是从茶（山茶科茶树）中提取的多酚类化合物，据报道具有广泛的生物活性，包括预防龋齿和口腔癌。本研究旨在确定绿茶和红茶提取物在将儿茶素和茶黄素输送到口腔中的效用。将绿茶叶（2克）或冲泡的红茶（2克茶叶溶于100毫升水中）含在口中2 - 5分钟，然后彻底漱口，在第1小时的唾液中观察到高浓度的儿茶素（C(max)=131.0 - 2.2微摩尔）和茶黄素（C(max)=1.8 - 0.6微摩尔）。尽管儿茶素和茶黄素的峰值水平存在显著的个体差异，但总体动力学曲线相似，儿茶素和茶黄素的t(1/2)分别为25 - 44分钟和49 - 76分钟（t(1/2)的平均变异系数为23.4%）。除了母体儿茶素和茶黄素峰外，红茶处理后的唾液中还观察到五个未鉴定的峰。在体外和体内均观察到茶黄素没食子酸酯明显被唾液酯酶水解。这些结果表明茶叶可作为儿茶素和茶黄素方便的缓释来源，并为茶在预防口腔癌和龋齿方面的可能应用提供了信息。
