兔青光眼滤过术后结缔组织生长因子的表达

Expression of connective tissue growth factor after glaucoma filtration surgery in a rabbit model.

作者信息

Esson Douglas W, Neelakantan Arvind, Iyer Sandhya A, Blalock Timothy D, Balasubramanian Lakshmi, Grotendorst Gary R, Schultz Gregory S, Sherwood Mark B

机构信息

Department of Ophthalmology, University of Florida, Gainesville, Florida 32610-0284, USA.

出版信息

Invest Ophthalmol Vis Sci. 2004 Feb;45(2):485-91. doi: 10.1167/iovs.03-0485.

DOI:10.1167/iovs.03-0485

PMID:14744889

Abstract

PURPOSE

Connective tissue growth factor (CTGF) appears to play a significant role in mediating fibrosis in several tissues. To gain further understanding of the role of CTGF in the scar formation that occurs after glaucoma filtering surgery (GFS), experiments were performed in a rabbit model.

METHODS

. Three experiments were performed: (1) CTGF and transforming growth factor (TGF)-beta expression were measured quantitatively after GFS, using ELISA. (2) After GFS conjunctival bleb tissues were immunostained for the presence of CTGF and TGF-beta. (3) Exogenous CTGF was injected into mitomycin-C (MMC)-treated filtering blebs and the scaring response compared to TGF-beta and physiological saline-injected blebs.

RESULTS

CTGF and TGF-beta were expressed maximally by day 5 after surgery and were both shown to be present in the bleb tissues after GFS. The addition of exogenous CTGF and TGF-beta increased the rate of failure of GFS blebs.

CONCLUSIONS

These data support the hypothesis that CTGF plays an important role in scarring and wound contracture after GFS. Inhibition of CTGF synthesis or its action may help prevent bleb failure and improve long-term GFS outcomes.

摘要

目的

结缔组织生长因子（CTGF）似乎在介导多种组织的纤维化过程中发挥重要作用。为了进一步了解CTGF在青光眼滤过手术（GFS）后瘢痕形成中的作用，我们在兔模型上进行了实验。

方法

进行了三项实验：（1）使用酶联免疫吸附测定法（ELISA）在GFS后定量测量CTGF和转化生长因子（TGF）-β的表达。（2）GFS后对结膜滤过泡组织进行免疫染色，检测CTGF和TGF-β的存在情况。（3）将外源性CTGF注入丝裂霉素C（MMC）处理过的滤过泡中，并与注入TGF-β和生理盐水的滤过泡的瘢痕形成反应进行比较。