Snodderly D Max, Mares Julie A, Wooten Billy R, Oxton Lisa, Gruber Michael, Ficek Tara
Schepens Eye Research Institute, Boston, Massachusetts, USA.
Invest Ophthalmol Vis Sci. 2004 Feb;45(2):531-8. doi: 10.1167/iovs.03-0762.
To develop a standardized protocol for measuring macular pigment optical density (MPOD) of experimentally naïve subjects by heterochromatic flicker photometry (HFP).
MPOD in eyes of 54 women, age 50 and 79 years (mean, 66), was studied. The spatial profile of MPOD was measured in the right eye, and two spatial points were also measured in the left eye. Forty-eight of these inexperienced subjects completed the protocol on two separate visits. For a subset of the group, the MPOD at two different wavelengths was measured.
The test-retest correlation at 0.5 degrees eccentricity in the right eye was 0.9. On the second visit, more than 90% of the subjects were able to perform the HFP test with results that were consistent with the absorption spectrum of macular pigment. On the first visit, data from the inexperienced subjects deviated more from the expected relationships between the two wavelengths, presumably because they had less skill in performing the task. However, subjects with high or low macular pigment density were distinguished clearly.
Reliable and meaningful measurements of macular pigment density in older subjects can be made using HFP, with a standardized protocol in the limited time available in large epidemiologic studies. This protocol will be made freely available to other researchers on request.
制定一种通过异色闪烁光度法（HFP）测量未进行过实验的受试者黄斑色素光密度（MPOD）的标准化方案。
对54名年龄在50至79岁（平均66岁）的女性的眼睛进行了MPOD研究。测量了右眼MPOD的空间分布，同时也测量了左眼的两个空间点。其中48名没有经验的受试者在两次单独的就诊中完成了该方案。对于该组中的一个子集，测量了两个不同波长下的MPOD。
右眼0.5度偏心率处的重测相关性为0.9。在第二次就诊时，超过90%的受试者能够完成HFP测试，其结果与黄斑色素的吸收光谱一致。在第一次就诊时，没有经验的受试者的数据与两个波长之间的预期关系偏差更大，可能是因为他们执行任务的技能较低。然而，黄斑色素密度高或低的受试者被明显区分开来。
在大型流行病学研究有限的时间内，使用标准化方案通过HFP可以对老年受试者的黄斑色素密度进行可靠且有意义的测量。该方案将根据要求免费提供给其他研究人员。
