Criswell Mark H, Ciulla Thomas A, Hill Tiffany E, Small Ward, Danis Ronald P, Snyder Wendy J, Lowseth Lisa A, Carson Dennis L
Retina Service Research Laboratories, Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana 46260, USA.
Invest Ophthalmol Vis Sci. 2004 Feb;45(2):625-34. doi: 10.1167/iovs.03-0718.
To evaluate and characterize the New-World squirrel monkey as a primate model for experimental choroidal neovascularization (CNV) studies and to compare it with the current Old-World macaque monkey model.
Fibrovascular tissues (FVT) were elicited in 12 maculae of seven squirrel monkeys by laser photocoagulation using optimized laser parameters (532 nm, 0.05 second, 75 micro m, 650 mW). Follow-up fundus and fluorescein angiography (FA) examinations were conducted on postlaser days 30 and 35, followed by euthanasia and histologic analysis of tissues. For comparative evaluations, FVT development also was induced and analyzed in eight maculae of four macaque monkeys with laser parameters previously used in this species (514 nm, 0.1 second, 50 micro m, 390 and 455 mW).
FVT developed in both primate species, consisting of fibrous tissue that contained vessels that ranged from sparse but identifiable capillaries to well-established neovascular networks. Overall, 65% of the photocoagulation sites in the squirrel monkey and 37% of sites in macaque monkey elicited development of FVT. Localized FVT ranged from modest to extensive thickenings of the choriocapillaris layer. Unexpectedly, 76% of the FVT sites in squirrel monkey eyes and 27% of the sites in macaque eyes showed diffuse FVT that expanded beyond the original photocoagulation sites, accompanied by neovascular infiltration of the retina.
Like the macaque, the squirrel monkey can be considered a useful primate model for experimental CNV investigations, while additionally offering certain species-specific advantages. Diffuse FVT permit studies of antiangiogenic therapies in areas distant from laser photocoagulative trauma sites.
评估和描述新大陆松鼠猴作为实验性脉络膜新生血管（CNV）研究的灵长类动物模型，并将其与现有的旧大陆猕猴模型进行比较。
使用优化的激光参数（532nm，0.05秒，75μm，650mW）对7只松鼠猴的12个黄斑进行激光光凝，诱发纤维血管组织（FVT）。在激光照射后第30天和第35天进行眼底和荧光素血管造影（FA）随访检查，随后实施安乐死并对组织进行组织学分析。为进行比较评估，还使用先前在该物种中使用的激光参数（514nm，0.1秒，50μm，390和455mW）对4只猕猴的8个黄斑诱发并分析FVT的发展情况。
两种灵长类动物均出现了FVT，其由纤维组织组成，其中包含从稀疏但可识别的毛细血管到成熟的新生血管网络不等的血管。总体而言，松鼠猴中65%的光凝部位和猕猴中37%的部位诱发了FVT的发展。局部FVT范围从脉络膜毛细血管层的轻度增厚到广泛增厚。出乎意料的是，松鼠猴眼中76%的FVT部位和猕猴眼中27%的部位显示出弥漫性FVT，其超出了原始光凝部位，并伴有视网膜新生血管浸润。
与猕猴一样，松鼠猴可被视为用于实验性CNV研究的有用灵长类动物模型，同时还具有某些物种特异性优势。弥漫性FVT允许在远离激光光凝创伤部位的区域研究抗血管生成疗法。
