Yasuda Shin, Wu Po-Sheng, Hattori Emi, Tachibana Hirofumi, Yamada Koji
Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):51-8. doi: 10.1271/bbb.68.51.
A method for simultaneous detection and quantification is presented to determine the presence of isoflavones and bisphenol A in a biological sample. A coulometric array detector was used with reversed-phase high-performance liquid chromatography (HPLC). Daidzein (1), glycitein (2), genistein (3) and their glucoside conjugates, daidzin (4), glycitin (5) and genistin (6), were measured as phytochemicals. Also assayed here was equol (7), a metabolite from compound 1, and bisphenol A (8), an industrial chemical that acts as an endocrine disrupter. All chemicals were simultaneously detected by using a 600-mV single detection voltage with high efficacy. A mixture of 1, 3 and 8 was orally administered to rats, and the levels of these three chemicals in the serum were clearly increased after a 4 kU beta-glucuronidase treatment. The levels of compounds 1 and 3 in the serum were detected at 1665 and 2040 ng/ml, while 8 was at a low level of 417 ng/ml. Compound 7 in the serum was not detected until after enzymatic hydrolysis (72 ng/ml). These results suggest that this analytical method would be useful for metabolic and pharmacokinetic studies on isoflavones and bisphenol A.
本文提出了一种同时检测和定量的方法，用于测定生物样品中异黄酮和双酚A的存在情况。采用库仑阵列检测器与反相高效液相色谱（HPLC）联用。大豆苷元（1）、染料木黄酮（2）、金雀异黄素（3）及其葡萄糖苷共轭物大豆苷（4）、染料木苷（5）和金雀异黄素苷（6）作为植物化学物质进行测定。这里还检测了雌马酚（7），它是化合物1的代谢产物，以及双酚A（8），一种作为内分泌干扰物的工业化学品。通过使用600 mV的单一检测电压高效地同时检测所有化学物质。将1、3和8的混合物口服给予大鼠，经4 kUβ-葡萄糖醛酸酶处理后，血清中这三种化学物质的水平明显升高。血清中化合物1和3的水平分别检测为1665和2040 ng/ml，而8的水平较低，为417 ng/ml。血清中的化合物7直到酶水解后才被检测到（72 ng/ml）。这些结果表明，这种分析方法将有助于异黄酮和双酚A的代谢和药代动力学研究。
