Zhao J H, Sun S J, Arao Y, Oguma E, Yamada K, Horiguchi H, Kayama F
Center for Community Medicine, Department of Environmental Medicine, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan.
Phytomedicine. 2006 May;13(5):304-9. doi: 10.1016/j.phymed.2005.09.013. Epub 2005 Oct 28.
Using a method of high-performance liquid chromatography (HPLC) with coulometric array, we measured isoflavone levels in sera from seven volunteers before and after three days of ingesting Soyaflavone E (an isoflavones powder) and from 129 female farmers (Japanese Multiple Environmental Toxicants Study; JMETS). Results showed that the serum isoflavone concentrations rose dramatically after three days of ingesting Soyaflavone E in all subjects except for the serum equol concentrations in two subjects. The geometric mean concentrations of daidzein, genistein, and equol in the serum of 129 Japanese women were 25.0 ng/ml of daidzein, 94.1 ng/ml of genistein, and 9.6 ng/ml of equol. Interestingly, there existed two dominant groups in terms of serum equol concentrations in an independent manner of soy-derived product intake among the study participants.
我们采用带库仑阵列的高效液相色谱法（HPLC），测量了7名志愿者在摄入大豆黄酮E（一种异黄酮粉末）三天前后血清中的异黄酮水平，以及129名女性农民（日本多重环境毒物研究；JMETS）血清中的异黄酮水平。结果显示，除两名受试者的血清雌马酚浓度外，所有受试者在摄入大豆黄酮E三天后血清异黄酮浓度均显著上升。129名日本女性血清中大豆苷元、染料木黄酮和雌马酚的几何平均浓度分别为25.0 ng/ml大豆苷元、94.1 ng/ml染料木黄酮和9.6 ng/ml雌马酚。有趣的是，在研究参与者中，血清雌马酚浓度存在两个主要组，且与大豆衍生产品的摄入量无关。
