担子菌香菇细胞色素P450（Le.CYP1酶）在酵母中产生的催化反应。
Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast.
作者信息
Akiyama Ryoko, Kajiwara Susumu, Shishido Kazuo
机构信息
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Biosci Biotechnol Biochem. 2004 Jan;68(1):79-84. doi: 10.1271/bbb.68.79.
The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.
担子菌香菇（Le.）细胞色素P450，即Le.CYP1，在酿酒酵母中实现了功能表达。从重组酵母中制备了含有Le.CYP1的微粒体部分，并对Le.CYP1进行了分析。发现7-乙氧基香豆素和苯并(a)芘是Le.CYP1酶的底物。Le.CYP1将7-乙氧基香豆素转化为7-羟基香豆素。
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