Kashima Yasuhiro, Udaka Shigezo
Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology (AIST Kansai), Osaka, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):235-7. doi: 10.1271/bbb.68.235.
A hyperthermophilic cellulase derived from Pyrococcus horikoshii was successfully produced with the Bacillus brevis host-vector system. The production of the recombinant enzyme was increased about 20-fold (to a level of 100 mg per liter) by the insertion of certain amino acid such as alanine and peptides like AEEAADP between the carboxyl end of signal peptide and the N-terminus of the mature cellulase. These recombinant cellulases had the same characteristics as that of the cellulase expressed in Escherichia coli.
利用短短芽孢杆菌宿主-载体系统成功生产了源自嗜热栖热菌的一种超嗜热纤维素酶。通过在信号肽羧基末端与成熟纤维素酶N端之间插入某些氨基酸（如丙氨酸）和肽（如AEEAADP），重组酶的产量提高了约20倍（达到每升100毫克的水平）。这些重组纤维素酶具有与在大肠杆菌中表达的纤维素酶相同的特性。
