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Introduction of DPR, an enterostatin fragment peptide, into soybean beta-conglycinin alpha' subunit by site-directed mutagenesis.

作者信息

Takenaka Yasuyuki, Doyama Naomi, Maruyama Nobuyuki, Utsumi Shigeru, Yoshikawa Masaaki

机构信息

Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

出版信息

Biosci Biotechnol Biochem. 2004 Jan;68(1):253-6. doi: 10.1271/bbb.68.253.

DOI:10.1271/bbb.68.253
PMID:14745196
Abstract

DPR, a fragment peptide of enterostatin (VPDPR) having hypocholesterolemic activity, was introduced into the three homologous sites, EPR, DYR, and DPI, in the soybean beta-conglycinin alpha' subunit by site-directed mutagenesis. The modified beta-conglycinin was expressed in Escherichia coli and recovered in the soluble fraction. After purification on ion-exchange HPLC, the modified beta-conglycinin was digested by trypsin to release integrated DPR. The yield of DPR from 1 mole of the modified beta-conglycinin was 1.2 mole.

摘要

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