Takenaka Y, Utsumi S, Yoshikawa M
Research Institute for Food Science, Kyoto University, Uji, Japan.
Biosci Biotechnol Biochem. 2000 Dec;64(12):2731-3. doi: 10.1271/bbb.64.2731.
Enterostatin (VPDPR), having anoretic and hypocholesterolemic activities, and its homologue LPYPR, a hypocholesterolemic peptide found in the glycinin A5A4B3 subunit, were introduced into the corresponding site (TNGPQ) of the proglycinin A1aB1b subunit by site-directed mutagenesis. Modified proglycinins were expressed in E. coli and recovered from the insoluble fraction. VPDPR and LPYPR were released by the action of chymotrypsin and trypsin as expected. The overall yields of purified VPDPR and LPYPR were 40% and 62%, respectively.
