Edelhofer Renate, Müller Astrid, Schuh M, Obritzhauser W, Kanout A
Institute for Parasitology and Zoology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
Parasitol Res. 2004 Mar;92(5):433-5. doi: 10.1007/s00436-003-1060-x. Epub 2004 Jan 27.
To establish an assay for the serological differentiation of bovine Babesia species ( B. bigemina, B. bovis, B. divergens and B. major), antigens from experimentally infected cattle were Western blotted and probed with homologous and heterologous sera. Varying antigen patterns for each species allowed the determination of species-specific diagnostic antigens. Blood samples from 36 naturally infected cattle from the province of Styria were tested by indirect immunofluorescence antibody test (IFAT) against B. divergens, as well as by Western blotting against B. bigemina, B. bovis, B. divergens and B. major, 3 weeks after clinical babesiosis was diagnosed by blood smears. All 36 cattle were B. divergens-positive when tested by IFAT. In four cases (11%), an infection with both B. bovis and B. divergens and in two cases a single infection with B. bovis were diagnosed when tested by Western blot. B. bigemina and B. major infections were not detected. These are the first serologically confirmed cases of B. bovis in Austrian cattle.
为建立一种用于牛巴贝斯虫物种（双芽巴贝斯虫、牛巴贝斯虫、分歧巴贝斯虫和大型巴贝斯虫）血清学鉴别诊断的检测方法，将来自实验感染牛的抗原进行蛋白质免疫印迹，并与同源和异源血清进行反应。每个物种呈现出不同的抗原模式，从而能够确定物种特异性诊断抗原。在通过血涂片确诊临床巴贝斯虫病3周后，采用间接免疫荧光抗体试验（IFAT）检测了施蒂里亚州36头自然感染牛的血液样本，以检测其对分歧巴贝斯虫感染情况，并采用蛋白质免疫印迹法检测其对双芽巴贝斯虫、牛巴贝斯虫、分歧巴贝斯虫和大型巴贝斯虫的感染情况。通过IFAT检测，所有36头牛均为分歧巴贝斯虫阳性。采用蛋白质免疫印迹法检测时，在4例（11%）中诊断出同时感染牛巴贝斯虫和分歧巴贝斯虫，2例诊断为单独感染牛巴贝斯虫。未检测到双芽巴贝斯虫和大型巴贝斯虫感染。这些是奥地利牛群中首例经血清学确诊的牛巴贝斯虫病例。
