National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan; Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Exp Parasitol. 2019 Nov;206:107758. doi: 10.1016/j.exppara.2019.107758. Epub 2019 Sep 12.
The diagnostic performance of a cocktail formula consisting of two Babesia (B.) bovis recombinant proteins, including spherical body protein 1 (BbSBP-1) and spherical body protein 4 (BbSBP-4), was evaluated in the present study for the global detection of B. bovis infection in cattle and for the differentiation between B. bovis and B. bigemina infections. The efficacy and the practicality of the rBbSBP-1 and rBbSBP-4 cocktail formula for differentiation between the infection caused by both parasites were assessed using indirect enzyme-linked immunosorbent assay (iELISA) with serum samples collected from cattle experimentally infected by B. bovis (n = 33) or B. bigemina (n = 30). Cocktail antigen exhibited the highest optical density (OD) values with B. bovis-infected sera and the lowest OD values with normal bovine sera or B. bigemina-infected sera in comparison with the single antigen. A total of 581 field serum samples collected from four countries with known B. bovis endemicity: Ghana (n = 154), Egypt (n = 162), Thailand (n = 96), and South Africa (n = 169) were screened also in the current study using iELISA and the results were compared to those of indirect fluorescent antibody test (IFAT) as a reference. A cocktail formula (rBbSBP-1 and rBbSBP-4) exhibited the highest concordance rate (89.90%) and kappa value (0.73). The obtained results revealed the reliability of the rBbSBP-1 and rBbSBP-4 cocktail antigen for the detection of specific antibodies to B. bovis in cattle and demonstrated the usefulness of cocktail antigen for differentiation between B. bovis and B. bigemina infections compared with the single antigen in cattle, which will be useful for epidemiological surveys and control of bovine babesiosis.
本研究评估了由两种巴贝斯虫（B.）重组蛋白组成的鸡尾酒配方（包括球形蛋白 1（BbSBP-1）和球形蛋白 4（BbSBP-4））在牛中的全球检测牛巴贝斯虫感染和区分牛巴贝斯虫和双芽巴贝斯虫感染的诊断性能。使用间接酶联免疫吸附试验（iELISA）评估了 rBbSBP-1 和 rBbSBP-4 鸡尾酒配方用于区分两种寄生虫感染的功效和实用性，血清样本来自通过 B. bovis（n = 33）或 B. bigemina（n = 30）实验感染的牛收集。与单抗原相比，鸡尾酒抗原在感染 B. bovis 的血清中表现出最高的光密度（OD）值，在正常牛血清或感染 B. bigemina 的血清中表现出最低的 OD 值。本研究还使用 iELISA 对来自四个已知存在 B. bovis 地方性流行的国家（加纳（n = 154）、埃及（n = 162）、泰国（n = 96）和南非（n = 169））收集的 581 份现场血清样本进行了筛选，并将结果与间接荧光抗体试验（IFAT）的结果进行了比较。鸡尾酒配方（rBbSBP-1 和 rBbSBP-4）显示出最高的一致性率（89.90%）和kappa 值（0.73）。结果表明，rBbSBP-1 和 rBbSBP-4 鸡尾酒抗原用于检测牛中针对 B. bovis 的特异性抗体是可靠的，并且与单抗原相比，鸡尾酒抗原用于区分牛巴贝斯虫和双芽巴贝斯虫感染在牛中具有实用性，这将有助于流行病学调查和牛巴贝斯虫病的控制。
