Nouhra Eduardo R, Horton Thomas R, Cazares Efren, Castellano Michael
IMBIV (CONICET/UNC), C.C. 495, 5000 Córdoba, Argentina.
Mycorrhiza. 2005 Jan;15(1):55-9. doi: 10.1007/s00572-004-0294-5. Epub 2004 Jan 27.
Ramaria species are conspicuous mycorrhizal symbionts of conifers in the Pacific Northwest. Here we collected and identified sporocarps and associated ectomycorrhizae of Ramaria acrisiccescens Marr & Stuntz, R. cyaneigranosa Marr & Stuntz, R. sandaracina Marr & Stuntz, R. celerivirescens Marr & Stuntz, and R. flavobrunnescens var. aromatica Marr & Stuntz. An internal transcribed spacer (ITS)- restriction fragment length polymorphism pattern was observed for each of the Ramaria species and used as a diagnostic tool to support the identification of mycorrhizae occurring in mats below the sporocarps. We provide a description of ectomycorrhizae of Ramaria, which exhibit similar macro- and microscopic characteristics such as ramification pattern, coloration, abundance of mycelial strands and emanating hyphae, mantle morphology and chemical reactions of mantle and mycelial strands with KOH, FeSO4 and Melzer's reagent. Sequences of the ITS region for each of the species are deposited in the GenBank.
枝瑚菌属物种是太平洋西北部针叶树显著的菌根共生体。在此，我们采集并鉴定了尖顶枝瑚菌（Marr & Stuntz）、蓝褐枝瑚菌（Marr & Stuntz）、黄枝瑚菌（Marr & Stuntz）、速绿枝瑚菌（Marr & Stuntz）以及芳香黄褐枝瑚菌变种（Marr & Stuntz）的子实体和相关外生菌根。观察了每个枝瑚菌属物种的内部转录间隔区（ITS）-限制性片段长度多态性模式，并将其用作诊断工具，以支持鉴定子实体下方菌垫中出现的菌根。我们对枝瑚菌属的外生菌根进行了描述，它们在宏观和微观特征上相似，如分支模式、颜色、菌丝束和发散菌丝的丰度、菌套形态以及菌套和菌丝束与氢氧化钾、硫酸亚铁和梅尔泽试剂的化学反应。每个物种的ITS区域序列已存入GenBank。
