Hu Wenqi, Cook Barbara J, Ampasala Dinakara R, Zheng Sichun, Caputo Guido, Krell Peter J, Retnakaran Arthur, Arif Basil M, Feng Qili
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada.
Arch Insect Biochem Physiol. 2004 Feb;55(2):68-78. doi: 10.1002/arch.10124.
The morphological and molecular responses of a midgut-derived cell line of the spruce budworm, Choristoneura fumiferana, to 20-hydroxyecdysone (20E) and the nonsteroidal ecdysone agonist, tebufenozide (RH-5992), were investigated. The cells responded to these compounds by clumping, generating filamentous extensions, increased mortality and expression of the transcription factor, Choristoneura hormone receptor 3 (CHR3). This cell line can be used as a model system to study the mode of action of ecdysone and its agonists. With subsequent passaging in ecdysteroid-containing medium, the degree of clumping increased and the clumping could not be reversed by subculturing in ecdysteroid-free medium. Cell numbers of the adapted cell lines in 20E and RH-5992 containing media were not significantly decreased, compared to the control, but both cell lines accumulated less (14)C-labeled RH-5992 and lost the capability of expressing CHR3 in response to these compounds. Taken together, the cell lines appeared to develop a mechanism to adapt to the toxic effects of these compounds. Arch. Insect Biochem. Physiol. 55:68-78, 2004.
对云杉芽卷叶蛾（Choristoneura fumiferana）中肠来源的细胞系，就其对20 - 羟基蜕皮酮（20E）和非甾体蜕皮酮激动剂虫酰肼（RH - 5992）的形态学及分子反应进行了研究。这些细胞对这些化合物的反应表现为成团、产生丝状延伸、死亡率增加以及转录因子云杉芽卷叶蛾激素受体3（CHR3）的表达增加。该细胞系可作为一个模型系统来研究蜕皮酮及其激动剂的作用模式。在含蜕皮甾类的培养基中传代培养后，成团程度增加，且在不含蜕皮甾类的培养基中继代培养无法逆转这种成团现象。与对照相比，在含20E和RH - 5992的培养基中适应后的细胞系细胞数量没有显著减少，但两个细胞系积累的（14）C标记的RH - 5992都减少了，并且失去了对这些化合物产生反应而表达CHR3的能力。综上所述，这些细胞系似乎形成了一种机制来适应这些化合物的毒性作用。《昆虫生物化学与生理学档案》55:68 - 78，2004年。
