Luhur Arthur, Mariyappa Daniel, Bohall Peter, Multini Laura, Elkins Morgan, Zelhof Andrew C
Drosophila Genomics Resource Center Biology Department, Indiana University, Bloomington, IN, 47405, USA.
Sci Rep. 2025 Sep 2;15(1):32291. doi: 10.1038/s41598-025-17336-z.
The Drosophila intestine is a powerful model for stem cell dynamics and epithelial biology, yet no intestinal derived cell lines have been available until now. Here, we describe the establishment of Drosophila intestinal cell lines. The cell lines were derived from the embryonic intestine through specific Ras expression and show continuous proliferation and the ability to be frozen and re-thawed. Each derived cell line exhibited morphological and cellular heterogeneity. Single-cell RNA sequencing confirmed their intestinal origin and cell populations with unique enriched signaling pathways. In addition, L15, one of the three lines, formed 3D spheroids that displayed epithelial polarity. Together, these lines provide an additional resource for studying intestinal development, epithelial organization, and pest management.
果蝇肠道是研究干细胞动力学和上皮生物学的有力模型，但迄今为止尚未有源自肠道的细胞系。在此，我们描述了果蝇肠道细胞系的建立。这些细胞系通过特定的Ras表达从胚胎肠道衍生而来，表现出持续增殖以及冷冻和解冻后仍能存活的能力。每个衍生的细胞系都表现出形态和细胞异质性。单细胞RNA测序证实了它们的肠道起源以及具有独特富集信号通路的细胞群体。此外，三个细胞系之一的L15形成了具有上皮极性的3D球体。总之，这些细胞系为研究肠道发育、上皮组织和害虫管理提供了额外的资源。
