苦瓜（Momordica charantia）种子中一种植物核糖核酸酶的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of a plant ribonuclease from the seeds of the bitter gourd Momordica charantia.

作者信息

De A, Funatsu G

机构信息

Protein Structure Laboratory, Imperial Cancer Research Fund, London, U.K.

出版信息

J Mol Biol. 1992 Dec 20;228(4):1271-3. doi: 10.1016/0022-2836(92)90334-g.

DOI:10.1016/0022-2836(92)90334-g

PMID:1474592

Abstract

Single crystals of ribonuclease Mc, a new class of plant ribonuclease from the seeds of the bitter gourd, were obtained from solutions of polyethylene glycol 8000 by the hanging-drop vapour diffusion method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1) with cell dimensions a = 67.28 A, b = 75.21 A, c = 38.54 A. The assumption of one monomer per asymmetric unit gives rise to a Vm value of 2.29 A3/Da. The crystals diffract beyond 2.0 A resolution and are suitable for high resolution X-ray structure analysis.

摘要

从苦瓜种子中提取的一种新型植物核糖核酸酶——核糖核酸酶Mc的单晶，通过悬滴气相扩散法从聚乙二醇8000溶液中获得。这些晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数为a = 67.28 Å，b = 75.21 Å，c = 38.54 Å。每个不对称单元假设为一个单体，Vm值为2.29 Å3/Da。这些晶体的衍射分辨率超过2.0 Å，适用于高分辨率X射线结构分析。