Crystallization and preliminary x-ray data for a glycosylated form of bovine pancreatic ribonuclease.

A glycosylated form of bovine pancreatic ribonuclease, ribonuclease B, has been crystallized in a form suitable for three-dimensional x-ray structure determination. Crystals grown from polyethylene glycol solutions display high resolution diffraction patterns indicating the orthorhombic space group P2(1)2(1)2(1) with a = 59.2 (+/- 0.1), b = 56.0 (+/- 0.1), and c = 81.0 (+/- 0.2). A, V = 2.7 x 10(5) A3. With 8 molecules/unit cell Vm = 2.28 A3/dalton. The systemically weak intensity of all reflections with their l indices odd suggests the likely alignment of a noncrystallographic 2-fold axis near the crystal c axis.