Nagae Y, Miyamoto M, Miyamoto H
Drug Safety Laboratory, Ciba-Geigy Ltd., Hyogo, Japan.
J Toxicol Sci. 1992 Nov;17(4):185-95. doi: 10.2131/jts.17.185.
The effect of estrogen on plasma membrane was investigated using the primary cultured rat hepatocytes treated with carbon tetrachloride (CCl4) and the isolated plasma membrane of rat liver. 17 beta-Estradiol (E2), at concentrations of 10(-10) M to 10(-4) M, 10(-8) M to 10(-6) M and 10(-12) M to 10(-4) M, had an inhibitory effect on the CCl4-induced leakage of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and lactate dehydrogenase, respectively from primary cultured rat hepatocytes. Diethylstilbestrol, which caused inhibition at a dose of 10(-4) M, did not inhibit any enzyme leakage at any further concentrations of 10(-12) M to 10(-6) M. In the isolated plasma membrane of rat liver, Mg(2+)- and Na+,K(+)-adenosine triphosphatase activity was increased by E2 treatment at concentrations of 10(-6) M and 10(-4) M.
使用经四氯化碳（CCl4）处理的原代培养大鼠肝细胞和分离的大鼠肝细胞膜，研究了雌激素对细胞膜的影响。17β-雌二醇（E2）浓度为10^(-10) M至10^(-4) M、10^(-8) M至10^(-6) M以及10^(-12) M至10^(-4) M时，分别对原代培养大鼠肝细胞中四氯化碳诱导的谷草转氨酶、谷丙转氨酶和乳酸脱氢酶泄漏具有抑制作用。己烯雌酚在剂量为10^(-4) M时产生抑制作用，但在10^(-12) M至10^(-6) M的任何其他浓度下均未抑制任何酶的泄漏。在分离的大鼠肝细胞膜中，浓度为10^(-6) M和10^(-4) M的E2处理可增加镁离子和钠钾离子三磷酸腺苷酶的活性。
