Yuasa S, Sudoh A, Nakao Y, Umezu K
Jpn J Pharmacol. 1986 Jun;41(2):205-10. doi: 10.1254/jjp.41.205.
Since tritoqualine (TRQ) is effective in suppressing the increase of serum transaminases in acute hepatic injured rats induced by some hepatotoxins, protection of the hepatocyte membrane is suggested to be one of the pharmacological effects of TRQ. In the present study, we investigated the effects of TRQ on lipid peroxidation and enzyme leakage caused by carbon tetrachloride (CCl4) exposure in isolated hepatocytes and the liver in vivo, compared with vitamin E. The results were as follows: Hepatocytes isolated from TRQ-administered rats showed less enzyme leakage than those from control rats after CCl4 addition. TRQ displayed strong inhibition of lipid peroxidation in isolated hepatocytes. In comparison with vitamin E, TRQ showed almost the same inhibitory action on lipid peroxidation, but a stronger suppression of enzyme leakage. Vitamin E showed a weaker protection from increase of glutamic oxaloacetic transaminase than TRQ, in spite of its stronger inhibition of lipid peroxidation in vivo. From these results, it is suggested that the membrane protecting action of TRQ is partially derived from its suppression of lipid peroxidation, but "another action" may also play an important role in protecting the fragile membrane.
由于三甲喹啉（TRQ）可有效抑制某些肝毒素诱导的急性肝损伤大鼠血清转氨酶的升高，因此推测保护肝细胞膜是TRQ的药理作用之一。在本研究中，我们研究了TRQ对离体肝细胞和体内肝脏中四氯化碳（CCl4）暴露引起的脂质过氧化和酶泄漏的影响，并与维生素E进行了比较。结果如下：添加CCl4后，从给予TRQ的大鼠分离出的肝细胞的酶泄漏比对照大鼠的少。TRQ对离体肝细胞中的脂质过氧化有强烈抑制作用。与维生素E相比，TRQ对脂质过氧化的抑制作用几乎相同，但对酶泄漏的抑制作用更强。尽管维生素E在体内对脂质过氧化的抑制作用更强，但它对谷氨酸草酰乙酸转氨酶升高的保护作用比TRQ弱。从这些结果可以看出，TRQ的膜保护作用部分源于其对脂质过氧化的抑制作用，但“另一种作用”在保护脆弱的膜方面可能也起着重要作用。
