[应用ATP检测法对泌尿生殖系统肿瘤进行化学敏感性试验的研究]

[Study on chemosensitivity test of urogenital tumors by ATP assay].

作者信息

Yamada S, Deguchi T, Nezasa S, Tamaki M, Ehara H, Okano M, Kawada Y

机构信息

Department of Urology, Gifu University School of Medicine.

出版信息

Nihon Hinyokika Gakkai Zasshi. 1992 Dec;83(12):2022-8. doi: 10.5980/jpnjurol1989.83.2022.

DOI:10.5980/jpnjurol1989.83.2022

PMID:1474710

Abstract

In order to improve the efficacy of chemotherapy against urogenital cancers, we developed a chemosensitivity test by measuring intracellular ATP in cancer cells. In the fundamental experiments using HeLa cells, a significant correlation was observed between intracellular ATP levels and numbers of viable cells. We employed 8 drugs, ADM, VCR, VLB, MTX, 5-FU, PEP, MMC, and CDDP for the assay Thirty-four renal tumors, 68 urothelial tumors and 19 testicular tumors were tested, and evaluable results were obtained in 25 specimens of renal tumors (76%) 55 specimens of urothelial tumors (80%) and 17 specimens of testicular tumors (98%). According to the ATP assay, renal tumors were sensitive to ADM and MMC, urothelial tumors to ADM, MMC and CDDP and testicular tumors to ADM and MMC. The ATP assay to determine the chemosensitivity to clinical specimens was sensitive and efficient. Thus the ATP assay could be applied for selection of anticancer drugs in the chemotherapy of urogenital cancers.

摘要

为提高化疗对泌尿生殖系统癌症的疗效，我们通过测量癌细胞内的三磷酸腺苷（ATP）开发了一种化学敏感性测试。在使用HeLa细胞的基础实验中，观察到细胞内ATP水平与活细胞数量之间存在显著相关性。我们采用阿霉素（ADM）、长春新碱（VCR）、长春碱（VLB）、甲氨蝶呤（MTX）、5-氟尿嘧啶（5-FU）、培美曲塞（PEP）、丝裂霉素（MMC）和顺铂（CDDP）8种药物进行检测。对34例肾肿瘤、68例尿路上皮肿瘤和19例睾丸肿瘤进行了检测，在25例肾肿瘤标本（76%）、55例尿路上皮肿瘤标本（80%）和17例睾丸肿瘤标本（98%）中获得了可评估的结果。根据ATP检测，肾肿瘤对ADM和MMC敏感，尿路上皮肿瘤对ADM、MMC和CDDP敏感，睾丸肿瘤对ADM和MMC敏感。用于确定临床标本化学敏感性的ATP检测灵敏且高效。因此，ATP检测可应用于泌尿生殖系统癌症化疗中抗癌药物的选择。