肠型和实体型胃癌肿瘤基质中缺乏血管外膜成纤维细胞。
Lack of vascular adventitial fibroblastic cells in tumour stroma of intestinal-type and solid-type gastric carcinomas.
作者信息
Nakayama H, Enzan H, Miyazaki E, Kuroda N, Toi M, Hiroi M, Yasui W
机构信息
Department of Molecular Pathology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
出版信息
J Clin Pathol. 2004 Feb;57(2):183-5. doi: 10.1136/jcp.2003.011312.
AIMS
To investigate the roles of vascular adventitial fibroblastic cells in tumour stroma, the distribution of vascular adventitial fibroblastic cells was studied in gastric carcinomas.
METHODS
In total, 50 surgically resected gastric carcinomas (43 intestinal type, and seven solid type) and their normal tissues were examined. Vascular adventitial fibroblastic cells are positive for CD34 but negative for CD31. To differentiate vascular adventitial fibroblastic cells from vascular endothelial cells, immunostaining for CD34 and CD31 was performed. Immunostaining for high molecular weight caldesmon was also performed to recognise vascular media.
RESULTS
In normal gastric tissues, CD34 positive fibroblastic cells were found just outside the vascular media, namely vascular adventitial fibroblastic cells. In contrast, all of the 43 intestinal-type and seven solid-type gastric carcinomas had no vascular adventitial fibroblastic cells in the tumour stroma.
CONCLUSIONS
These results suggest that a lack of vascular adventitial fibroblastic cells is associated with tumour stroma formation in intestinal-type and solid-type gastric carcinomas.
目的
为研究血管外膜成纤维细胞在肿瘤基质中的作用，对胃癌组织中血管外膜成纤维细胞的分布进行研究。
方法
共检查了50例手术切除的胃癌（43例肠型，7例实体型）及其正常组织。血管外膜成纤维细胞CD34呈阳性，但CD31呈阴性。为了区分血管外膜成纤维细胞与血管内皮细胞，进行了CD34和CD31免疫染色。还进行了高分子量钙调蛋白免疫染色以识别血管中膜。
结果
在正常胃组织中，CD34阳性成纤维细胞位于血管中膜外侧，即血管外膜成纤维细胞。相比之下，43例肠型和7例实体型胃癌的肿瘤基质中均无血管外膜成纤维细胞。
结论
这些结果表明，缺乏血管外膜成纤维细胞与肠型和实体型胃癌的肿瘤基质形成有关。
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